Regulatory

Part:BBa_K2447501:Design

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-12)


Blue light activated inducible system with RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal AgeI site found at 1473
    Illegal AgeI site found at 1585
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Jayaraman P. et al. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. https://www.ncbi.nlm.nih.gov/pubmed/27353329

References